Simultaneous Measurement of Nitrogen Fixation Estimated by Acetylene-Ethylene Assay and Nitrate Absorption by Soybeans
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منابع مشابه
Simultaneous measurement of nitrogen fixation estimated by acetylene-ethylene assay and nitrate absorption by soybeans.
An apparatus was designed for simultaneous measurement of rates of N(2) fixation estimated by C(2)H(2)-C(2)H(4) assay (N(2)[C(2)H(2)] fixation) and NO(3) (-) absorption by roots of intact, nodulated soybeans (Glycine max [L.] Merr.). The principal design features include: (a) a gas-tight mist chamber in which nodulated roots can be exposed simultaneously to C(2)H(2) in the gas phase and to a li...
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The effect of nitrate on symbiotic nitrogen fixation by root nodules of cowpea (Vigna unguiculata L., Walp., cv. California Blackeye) and lupine (Lupinus augustifolius L., cv. Frost) plants inoculated with nitrate reductase-expressing and nitrate reductase-nonexpressing Rhizobium strains were examined. Nitrate reductase of Rhizobium bacteroids in the nodules of cowpea and lupine reduced nitrate...
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The methodology, characteristics and application of the sensitive C(2)H(2)-C(2)H(4) assay for N(2) fixation by nitrogenase preparations and bacterial cultures in the laboratory and by legumes and free-living bacteria in situ is presented in this comprehensive report. This assay is based on the N(2)ase-catalyzed reduction of C(2)H(2) to C(2)H(4), gas chromatographic isolation of C(2)H(2) and C(2...
متن کاملThe acetylene reduction assay for measuring nitrogen fixation in waterlogged soil.
Nitrogen fixation in waterlogged, soil-straw, and sandday-straw mixtures was measured with the C2H2reduction assay, the I5N-tracer technique, and the Kjeldahl method. The reduction of 6 to 15 moles C2H2 corresponded to the fixation of 1 mole N2. The theoretical ratio is 3 moles C2H2 to 1 mole N2. A ratio of 3 moles C2H2 reduced for each mole of N2 fixed was obtained when samples of sandxlay-str...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1978
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.62.3.443